Relative abundance of microbial populations and amount of rRNA extracted from ruminal and dual-flow continuous culture fermenter samples

Range Table - link
Organism Microbes
Reference Ziemer CJ et al., Comparison of microbial populations in model and natural rumens using 16S ribosomal RNA-targeted probes. Environ Microbiol. 2000 Dec2(6):632-43. p.634 table 1PubMed ID11214796
Method Abstract: "A model rumen system, dual-flow continuous culture fermenters, was evaluated by two comparative criteria in two experiments using ribosomal (r)RNA-targeted DNA probes to compare key microbial groups in samples. The initial experiment measured temporal changes in population structure during adaptation of ruminal microbial populations in fermenters over 240 h. The fermenter inoculum contained 34.9% Bacteria, 60.1% Eukarya and 6.8% Archaea measured as a fraction of total small subunit (SSU) rRNA quantified using a universal probe."
Comments P.633 right column bottom paragraph: "The amount of SSU [small subunit] rRNA extracted (µg rRNA/g sample) and the relative abundance of microbial populations for ruminal and fermenters (0 and 240 h) are presented in Table 1." P.636 left column: "The total amount of SSU rRNA extracted from all samples was relatively consistent for both studies, averaging 163.4 µg/g of sample in the adaptation experiment and 164.7 µg/g of sample in the comparison experiment. In both experiments, inoculum samples had lower amounts of SSU rRNA extracted (0 h, Table 1 and Inoculum, Table 3)."
Entered by Davidlee
ID 112832