Metabolite pool sizes

Range Table - link
Organism Thale cress Arabidopsis thaliana
Reference Ma F, Jazmin LJ, Young JD, Allen DK. Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation. Proc Natl Acad Sci U S A. 2014 Nov 25 111(47):16967-72. doi: 10.1073/pnas.1319485111. Supporting Information p.8 of 8 table S1PubMed ID25368168
Method Abstract: "[Investigators] performed in vivo isotopic labeling of Arabidopsis thaliana rosettes with (13)CO2 and estimated fluxes throughout leaf photosynthetic metabolism by INST-MFA [Isotopically nonstationary metabolic flux analysis]. Plants grown at 200 µmol m^(-2)s^(-1) light were compared with plants acclimated for 9 d at an irradiance of 500 µmol⋅m^(-2)⋅s^(-1)."
Comments P.16970 left column 5th paragraph: "Despite the increase in photorespiration, the relative flux to sucrose export also increased to support more biomass production in HL-ACC [high light acclimated] plants. Sucrose export flux more than doubled from 11.7 to 26.4 µmol (hexose units)⋅gFW^−1⋅hr^−1, whereas starch production increased marginally from 6.3 to 8.5 µmol (hexose units)⋅gFW^−1⋅hr^−1. Higher carryover starch levels were observed in HL-ACC leaves throughout the diurnal cycle (Fig. S1). HL-ACC plants also had elevated levels of RuBisCO on the basis of leaf area, FW, or chlorophyll and produced thicker leaves with more biomass (Fig. S1). Furthermore, HL-ACC plants produced more seed biomass (i.e., 10 plants produced approximately twice the amount of seeds that resulted in 93% more biomass by weight) with a greater amount of oil, had altered leaf chlorophyll levels (Fig. S1), and had reduced measured concentrations of several of the Calvin cycle intermediates (Table S1)." P.16971 left column bottom paragraph: "This study also illustrates how combined analysis of flux and metabolite profiling data can provide complementary information about cellular reprogramming in response to light. For example, several measured metabolite intermediates appeared to adjust to the long-term high light acclimation through decreased pool sizes (on either a FW or chlorophyll basis) within the CBB [Calvin–Benson–Bassham] cycle (Table S1), even as their interconnected pathway fluxes increased. Although this result was unanticipated, it was consistently obtained in multiple experiments by using sample collection strategies specifically designed to minimize shading or other potential artifacts." LL=Low Light
Entered by Uri M
ID 112798