Bio-distribution and quantitative results of infused 14C-TdR [carbon radioisotope labeling thymidine] labeled PDB-MSCs [human placental deciduas basalis derived mesenchymal stem cells]
| Range | Figure - link |
|---|---|
| Organism | Mouse Mus musculus |
| Reference | Wu CG et al., In vivo tracking of human placenta derived mesenchymal stem cells in nude mice via ¹⁴C-TdR labeling. BMC Biotechnol. 2015 Jun 13 15: 55. doi: 10.1186/s12896-015-0174-4. p.5 fig.3PubMed ID26070459 |
| Method | Abstract: "In order to shed light on the regenerative mechanism of mesenchymal stem cells (MSCs) in vivo, the bio-distribution profile of implanted cells using a stable and long-term tracking method is needed. [Investigators] herein investigated the bio-distribution of human placental deciduas basalis derived MSCs (termed as PDB-MSCs) in nude mice after intravenous injection by carbon radioisotope labeling thymidine ((14)C-TdR), which is able to incorporate into new DNA strands during cell replication." |
| Comments | P.3 left column bottom paragraph: "The number of infused cells in each organ on day 30 was calculated according to average radioactivity of infused cells. As shown in Fig. 3b, the left femur harvested with bone marrow had the highest number of infused PDB-MSCs per gram (41,725 ± 2394/g) and the number of cells in lung, spleen, stomach, and liver detected on day 30 were 32,748 ± 3586, 16,031 ± 5640, 10,763 ± 777, 7557 ± 207 per gram of tissue, respectively. As two mice died because of the feeding machine accident, only one mouse was used for organs collection on day 180. The radioactivity in the organs were mainly detected in liver (1.08 dpm/mg), lung (3.82 dpm/mg) and left femur harvested with bone marrow (5.31 dpm/mg), indicating that the infused PDB-MSCs were able to exist in bone tissue up to 6 months (Fig. 3a)." See notes beneath figure |
| Entered by | Uri M |
| ID | 112713 |