Lifespan of long-term self-renewing hematopoietic stem cells (LT-HSC)

Value ~57 Days
Organism Mouse Mus musculus
Reference Cheshier SH, Morrison SJ, Liao X, Weissman IL. In vivo proliferation and cell cycle kinetics of long-term self-renewing hematopoietic stem cells. Proc Natl Acad Sci U S A. 1999 Mar 16 96(6):3120-5. abstract, p.3123 left column 2nd paragraph and p.3125 left column top paragraphPubMed ID10077647
Method Study of BrdUrd (Bromodeoxyuridine) incorporation kinetics
Comments "The potential doubling time of asynchronously dividing cells is the amount of time necessary for 77% of cells to incorporate BrdUrd (ref 25). Thus, the potential doubling time of all MPP [multipotent progenitors] and LT-HSC was 7.7 days and 17.8 days, respectively. Finally, the 99% BrdUrd-incorporation point was extrapolated from the log-linear plot as about 57 days for LT-HSC." "[Researchers'] data demonstrate that LTHSC enter the cell cycle in an asynchronous manner, that proliferation was not dominated by a few clones, and that on average 99% of LT-HSC divide every 57 days."
Entered by Uri M
ID 109232