Average length of primer in bacteria and eukaryotes
| Range | ~10 bp |
|---|---|
| Organism | Various |
| Reference | Michael Lynch and Georgi K. Marinov, The bioenergetic costs of a gene, PNAS 2015 doi: 10.1073/pnas.1514974112 link Supplementary Materials p.2 left column top paragraph |
| Primary Source | Zechner EL, Wu CA, Marians KJ. 1992. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. II. Frequency of primer synthesis and efficiency of primer utilization control Okazaki fragment size. J Biol Chem 267(6):4045-4053. & Chapados BR, Chai Q, Hosfield DJ, Qiu J, Shen B, Tainer JA. 2001. Structural biochemistry of a type 2 RNase H: RNA primer recognition and removal during DNA replication. J Mol Biol 307(2):541-556. & Rossi ML, Bambara RA. 2006. Reconstituted Okazaki fragment processing indicates two pathways of primer removal. J Biol Chem 281(36):26051-26061.PubMed ID1740452, 11254381, 16837458 |
| Comments | Supplementary Materials p.2 left column top paragraph:"Assuming Lof [Length of Okazaki Fragment] ~1500 bp for bacteria [BNID 110937, 102051] and 165 bp for eukaryotes [BNID 112149], and noting that RNA primers are on the order of 10 bp in both groups [primary sources], the expected costs of chain elongation for a gene of length Lg are then 0.32 LgPs in bacteria and 2.9 LgPs in eukaryotes." Ps=phosphate bonds hydrolyzed |
| Entered by | Uri M |
| ID | 112150 |