Cost of opening an origin of replication [ORI]

Range ≤20 ~P (ATP)
Organism Unspecified
Reference Michael Lynch and Georgi K. Marinov, The bioenergetic costs of a gene, PNAS 2015 doi: 10.1073/pnas.1514974112 link Supplementary Materials p.2 left column 2nd paragraph
Primary Source Lehninger AL, Nelson DL, Cox MM. 1993. Principles of Biochemistry, 2nd Edition. Worth Publishers, NY.
Comments Supplementary Materials p.2 left column 2nd paragraph:"Two other costs are being ignored here based on their likely minor contributions. First, the opening of an origin of replication requires perhaps as many as 20ATP [primary source], but given the large distance between ORIs, genes will typically either be devoid of ORIs entirely or harbor just one or two, so the total cost per base is very low. Second, assembly of the sliding clamp associated with the polymerase appears to require two to four additional ATP per event [refs 27, 28] focusing just on Okazaki fragments for reasons noted above, this implies an additional investment of ~3Lg/Lof P per gene, or 0.002 Lg P in bacteria and 0.018Lg P in eukaryotes, which is negligible relative to the investment in primers." Lg=gene Length, Lof=Length of Okazaki Fragment, Ps=phosphate bonds hydrolyzed
Entered by Uri M
ID 112151