| Comments |
P.368 right column bottom paragraph to p.370 left column top paragraph:"Assuming that the total concentrations of G-actin, profilin, and thymosin-β4 are 100, 10, and 200 μM (BNID 112134), respectively, the cellular concentrations of free G-actin, profilin- and thymosin-β4–bound G-actin, and free barbed and pointed ends of actin filaments under unstimulated conditions were estimated as 1.98 μM, 9.52 μM, 88.5 μM, 4.8 nM, and 5.5 nM, respectively, from the best-fit of the time curve of relative
FDAP (fluorescence decay after photoactivation, see Materials and methods). Thus, the high time resolution and quantitative features of s-FDAP [sequential FDAP] analysis allowed [investigators] to estimate the cellular concentrations of key components involved in the regulation of actin dynamics by model analysis." |