Fraction of total actin in polymorphonuclear neutrophil (PMN) that is in F (filamentous)-actin and G-actin state after chemoattractant stimulation

Range F-actin 60% - 70% or ~200µM: G-actin 30% - 40% or 100µM
Organism Unspecified
Reference Southwick FS, Young CL. The actin released from profilinactin complexes is insufficient to account for the increase in F-actin in chemoattractant-stimulated polymorphonuclear leukocytes. J Cell Biol. 1990 Jun110(6):1965-73. p.1965 left columnPubMed ID2351690
Comments P.1965 left column: "In unstimulated PMN, a high percentage of the total actin (60-70% or ~200µM, assuming a uniform distribution) is in an unpolymerized state. After chemoattractant stimulation there is rapid filament assembly, F-actin content reaching a maximum (60-70% of total actin, 200µM) and G-actin content reaching a nadir (30-40 % of total actin, 100µM) within 30-60 s. These observations indicate that the high concentrations of unpolymerized actin in resting PMNs are capable of being incorporated into filaments following stimulation."
Entered by Uri M
ID 112782