Diffusion coefficient of G-actin in cytoplasm

Range 3 - 30 μm^2/s
Organism Mammals
Reference Kiuchi T, Nagai T, Ohashi K, Mizuno K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J Cell Biol. 2011 Apr 18 193(2):365-80. doi: 10.1083/jcb.201101035. p.368 left column top paragraphPubMed ID21502360
Primary Source McGrath, J.L., Y. Tardy, C.F. Dewey Jr., J.J. Meister, and J.H. Hartwig. 1998. Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells. Biophys. J. 75: 2070–2078. doi:10.1016/S0006-3495(98)77649-0 & Zicha, D., I.M. Dobbie, M.R. Holt, J. Monypenny, D.Y.H. Soong, C. Gray, and G.A. Dunn. 2003. Rapid actin transport during cell protrusion. Science. 300:142–145. doi:10.1126/science.1082026 & McDonald, D., G. Carrero, C. Andrin, G. de Vries, and M.J. Hendzel. 2006. Nucleoplasmic beta-actin exists in a dynamic equilibrium between lowmobility polymeric species and rapidly diffusing populations. J. Cell Biol. 172:541–552. doi:10.1083/jcb.200507101PubMed ID9746549, 12677069, 16476775
Comments P.367 right column:"...The diffusion coefficient (13.7 μm^2/s) was consistent with the reported values for G-actin in the cytoplasm, which ranged from 3 to 30 μm^2/s (primary sources). These results indicate that single FDAP [fluorescence decay after photoactivation] analysis is a useful tool for estimating G-actin concentration in living cells. Based on the half-life of the mobile fraction (41 ms), [investigators] set the time point for image acquisition for s-FDAP analysis as 40ms after photoactivation." 1st primary source studied subconfluent bovine aortic endothelial cells (BAECs). 2nd primary source studied rat fibroblasts. 3rd primary source studied HeLa cells.
Entered by Uri M
ID 112133