| Range |
~11 %
|
| Organism |
Human Homo sapiens |
| Reference |
Clague MJ, Heride C, Urbé S. The demographics of the ubiquitin system. Trends Cell Biol. 2015 Apr 20. pii: S0962-8924(15)00054-9. doi: 10.1016/j.tcb.2015.03.002. p.2 right column bottom paragraph & p.3 left column top paragraphPubMed ID25906909
|
| Primary Source |
Kaiser, S.E. et al. (2011) Protein standard absolute quantification (PSAQ) method for the measurement of cellular ubiquitin pools. Nat. Methods 8, 691–696 doi: 10.1038/nmeth.1649.PubMed ID21743460
|
| Comments |
p.2 right column bottom paragraph:"Trypsin cleavage of ubiquitin chains (other than linear chains) renders a GlyGly C-terminal peptide linked through an isopeptide bond to a lysine residue in a substrate peptide. There-fore, when ubiquitin chains ( ~11% of the total ubiquitin pool in HEK293 cells [primary source]) are trypsin treated, specific diGly signature peptides for each chain linkage type are generated [ref 18]." |
| Entered by |
Uri M |
| ID |
111563 |