Fraction of total HeLa cellular protein that is the aggregate of ubiquitin, conjugating enzymes (E1, E2, and E3s), and deubiquitylases (DUBs)

Range ~1.3 %
Organism Eukaryotes
Reference Clague MJ, Heride C, Urbé S. The demographics of the ubiquitin system. Trends Cell Biol. 2015 Apr 20. pii: S0962-8924(15)00054-9. doi: 10.1016/j.tcb.2015.03.002. abstract & p.1 left column bottom paragraphPubMed ID25906909
Primary Source Kulak, N.A. et al. (2014) Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells. Nat. Methods 11, 319–324 doi: 10.1038/nmeth.2834.PubMed ID24487582
Comments p.1 left column bottom paragraph:"For the purposes of this review, [investigators] define the ubiquitin system as the complement of proteins directly involved in ubiquitin ligation, deubiquitylation, and ubiquitin itself, leaving aside the specific substrates and those proteins that bind to them. One can think of it as a system that converts the input of free ubiquitin into a variable set of conjugated forms. In HeLa cells, the aggregate of ubiquitin system proteins is estimated to be ~1.3% of the total cellular protein (extracted from [primary source])."
Entered by Uri M
ID 111553