| Range |
Table - link
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Soboll S, Scholz R, Heldt HW. Subcellular metabolite concentrations. Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of perfused rat liver. Eur J Biochem. 1978 Jun 15 87(2):377-90. p.383 table 4PubMed ID668699
|
| Method |
“From marker enzyme activities and metabolite
contents in the unfractionated homogenate and in
the different fractions the mitochondrial and cytosolic
metabolite contents (nmol/mg protein) were calculated
by extrapolating the data to fractions of pure mitochondria
and pure cytosol according to Elbers et al.
[ref 5]. The subcellular metabolite contents were converted
into concentrations assuming a water content of 0.8 and 3.8 µl/mg protein in the mitochondrial and
cytosolic compartment, respectively, based on considerations
previously discussed by Soboll et al. [ref 1].” |
| Comments |
"When the freeze-dried liver tissue was separated into mitochondrial and cytosolic fractions, only 20 - 27 of the overall tissue content of adenine nucleotides were found in the mitochondria (Table 4, right column). The observation of a non-uniform distribution of adenine nucleotides between mitochondria and cytosol confirms earlier studies with isolated hepatocytes and perfused liver [refs 1,5,16,17]." For experimental conditions and metabolic states see p.381 table 1 link |
| Entered by |
Uri M |
| ID |
111356 |