| Range |
nonreplicating genomic RNA 1μg/g leaf tissue: replicating genome 1mg/g leaf tissue
|
| Organism |
Turnip yellow mosaic virus (TYMV) |
| Reference |
Rao AL. Genome packaging by spherical plant RNA viruses. Annu Rev Phytopathol. 2006 44: 61-87. p.81 left columnPubMed ID16480335
|
| Method |
"To study the requirements for TYMV RNA
encapsidation, TYMV CP [coat protein] was expressed
in
trans
from a specialized mRNA, while a nonreplicating gRNA with authentic 5'
and 3'
termini was separately expressed." |
| Comments |
"The nonreplicating genome had a mutation in the active site of the RNA-dependent RNA polymerase. Although the nonreplicating gRNA
was present at much lower levels (1µg/g leaf
tissue) than the replicating genome (1mg/g leaf tissue), most of the RNA present in leaves
was encapsidated." |
| Entered by |
Uri M |
| ID |
111226 |