RNA amount often extracted from one mammalian cell

Range 0.1-1 pg
Organism Mammalian tissue culture cell
Reference Ginsberg SD. RNA amplification strategies for small sample populations. Methods. 2005 Nov37(3):229-37. p.229 right column bottom paragraphPubMed ID16308152
Primary Source J.E. Kacharmina, P.B. Crino, J. Eberwine, Preparation of cDNA from single cells and subcellular regions. Methods Enzymol. 303 (1999) 3–18. AND J. Phillips, J.H. Eberwine, Antisense RNA Amplification: A Linear Amplification Method for Analyzing the mRNA Population from Single Living Cells, Methods Enzymol. Suppl. 10 (1996) 283– 288. AND J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 2001.PubMed ID10349635, 8954839
Method (Methods mentioned in 1st 2 primary sources:) "Phenotypic characterization of cells in conjunction with single-cell mRNA analysis" AND "Patch clamp electrodes containing reverse transcriptase, dNTPS, and a poly(T) primer modified 5' with a T7 RNA polymerase promoter sequence are used to isolate the cytoplasmic contents of individual living cells."
Comments "Unfortunately, the quantity of RNA harvested from a single cell, estimated to be approximately 0.1–1.0pg, is not sufficient for standard RNA extraction procedures [primary sources]."
Entered by Kobi Benenson
ID 101740