| Value |
1.3e-3
substitution/bp
|
| Organism |
Yeast |
| Reference |
Imashimizu M, Oshima T, Lubkowska L, Kashlev M. Direct assessment of transcription fidelity by high-resolution RNA sequencing. Nucleic Acids Res. 2013 Oct41(19):9090-104. doi: 10.1093/nar/gkt698.PubMed ID23925128
|
| Primary Source |
Nesser,N.K., Peterson,D.O. and Hawley,D.K. (2006) RNA polymerase II subunit Rpb9 is important for transcriptional fidelity in vivo. Proc. Natl Acad. Sci. USA, 103, 3268–3273.PubMed ID16492753
|
| Method |
Primary source abstract:"The fidelity of yeast RNA polymerase II (Pol II) was assessed in vivo with an assay in which errors in transcription of can1–100, a nonsense allele of CAN1, result in enhanced sensitivity to the toxic arginine analog canavanine." |
| Comments |
"In eukaryotic transcription, Nesser et al. previously
analyzed transcription errors throughout ~450-bp
cDNA of CAN1 transcript in yeast by Sanger sequencing
(primary source). Their work reported a much higher 1.3×10^-3/bp rate
of substitutions compared to the rate observed for E. coli
RNAP in [researchers'] study and the rates determined for yeast
RNAP II in vitro (Kireeva et al. 2008 PMID 18538654)." |
| Entered by |
Uri M |
| ID |
111145 |