Error rate of RNA pol III determined solely by selectivity

Value 1.8e-4 correct/incorrect nucleotide
Organism Budding yeast Saccharomyces cerevisiae
Reference Alic N, Ayoub N, Landrieux E, Favry E, Baudouin-Cornu P, Riva M, Carles C. Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription. Proc Natl Acad Sci U S A. 2007 Jun 19 104(25):10400-5 abstractPubMed ID17553959
Method P.10400 right column bottom paragraph: "In this study, [investigators] examined nucleotide misincorporation by cleavage-competent and cleavage-deficient S. cerevisiae Pol III ternary complexes at a specific position on a tRNA gene. The results define the strategies used by a eukaryotic RNA Pol with high intrinsic cleavage activity to ensure the accuracy of its transcription. [They] used computational methods to combine the experimental observations and predict the steady-state error rate of Pol III and to demonstrate the equally high contribution of proofreading and nucleotide selectivity to the overall fidelity of Pol III."
Comments Abstract: "Determination of relative rates of the reactions producing correct and erroneous transcripts at a specific position on a tRNA gene, combined with computational methods, demonstrated that Pol III has a highly efficient proofreading activity increasing its transcriptional fidelity by a factor of 10^3 over the error rate determined solely by selectivity (1.8×10^-4)." See BNID 103453, 105213
Entered by Uri M
ID 105213