| Range |
>22,000 kDa
|
| Organism |
Fission yeast Schizosaccharomyces pombe |
| Reference |
Vavylonis D, Wu JQ, Hao S, O'Shaughnessy B, Pollard TD. Assembly mechanism of the contractile ring for cytokinesis by fission yeast. Science. 2008 Jan 4 319(5859):97-100. p.98 left column top paragraphPubMed ID18079366
|
| Primary Source |
Wu JQ et al., Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. J Cell Biol. 2006 Jul 31 174(3):391-402.PubMed ID16864655
|
| Method |
"[Researchers] used fluorescence microscopy of live fission yeast cells to observe that membrane-bound nodes containing myosin were broadly distributed around the cell equator and assembled into a contractile ring through stochastic motions, after a meshwork of dynamic actin filaments appeared." |
| Comments |
"When first formed, nodes moved relatively
little for ~10 min (Movies S5 and S6), but the
mean square displacement (MSD) of the center of each node increased over time (Fig. 2E, black),
consistent with 2-dimensional diffusion: MSD(t) =
MSD(0) + 4Dt. The diffusion constants D distributed
around 20 nm^2/s in control cells and cells
treated with Latrunculin A to depolymerize actin
(fig. S2). Node motions appeared uncorrelated,
because the slopes of relative MSD between
pairs of nodes (Fig. 2E, gray, and fig. S2B) were
on average equal to the sum of the slopes of
individual node MSD curves. Diffusive behavior
implies that any anchors or cross-links of
nodes were dynamic or very soft on minute time
scales. The large mass [>22,000 kD (primary source)] of
nodes may contribute to D being orders of magnitude
smaller than D of transmembrane proteins
(13)." |
| Entered by |
Uri M |
| ID |
111110 |