Width of band of membrane-associated nodes that condense to form cytokinetic contractile ring

Range ~1.8 µm
Organism Fission yeast Schizosaccharomyces pombe
Reference Vavylonis D, Wu JQ, Hao S, O'Shaughnessy B, Pollard TD. Assembly mechanism of the contractile ring for cytokinesis by fission yeast. Science. 2008 Jan 4 319(5859):97-100. p.97 right column 3rd paragraphPubMed ID18079366
Method "[Researchers] used fluorescence microscopy of live fission yeast cells to observe that membrane-bound nodes containing myosin were broadly distributed around the cell equator and assembled into a contractile ring through stochastic motions, after a meshwork of dynamic actin filaments appeared."
Comments "Fission yeast assembled a cytokinetic contractile ring by condensation of a broad band of membrane-associated nodes (Fig. 1, A and B, and Movie S1)...Wild-type cells assembled 63 ± 10 (n = 22) nodes located close to the plasma membrane in a band ~1.8µm wide around the middle of the cell (Fig. 1A and Movie S1). Nodes were distributed randomly around the equator and in a Gaussian manner along the long axis of the cell (fig. S1A)."
Entered by Uri M
ID 111108