Number of membrane-associated nodes that condense to form cytokinetic contractile ring

Value 63 nodes/cell Range: ±10 nodes/cell
Organism Fission yeast Schizosaccharomyces pombe
Reference Vavylonis D, Wu JQ, Hao S, O'Shaughnessy B, Pollard TD. Assembly mechanism of the contractile ring for cytokinesis by fission yeast. Science. 2008 Jan 4 319(5859):97-100. p.97 right column 3rd paragraphPubMed ID18079366
Method "[Researchers] used fluorescence microscopy of live fission yeast cells to observe that membrane-bound nodes containing myosin were broadly distributed around the cell equator and assembled into a contractile ring through stochastic motions, after a meshwork of dynamic actin filaments appeared."
Comments "Fission yeast assembled a cytokinetic contractile ring by condensation of a broad band of membrane-associated nodes (Fig. 1, A and B, and Movie S1)...Wild-type cells assembled 63 ± 10 (n = 22) nodes located close to the plasma membrane in a band ~1.8µm wide around the middle of the cell (Fig. 1A and Movie S1). Nodes were distributed randomly around the equator and in a Gaussian manner along the long axis of the cell (fig. S1A)."
Entered by Uri M
ID 111107