| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Shearer G, Lee JC, Koo JA, Kohl DH. Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli. FEBS J. 2005 Jul272(13):3260-9. p.3262 table 1PubMed ID15978033
|
| Method |
"In this study, [researchers] describe experiments aimed at calculating
the fraction of the flux from early glycolytic
intermediates to CO2 in intact Escherichia coli. Cells
incubated with [14C]glucose made 14C-labeled glycolytic
intermediates. When the incubation mix also
included a [12C]intermediate, assuming that this intermediate
entered the cell, there was a competition
between the intermediate just produced in the pathway
and the same molecular species in the aqueous cytosol.
To the degree the latter was successful, the amount of
14CO2 evolved was decreased. Cells were also incubated
in [12C]glucose plus [14C]intermediate, [14C]glucose
alone and [14C]intermediate alone. Combining these
data allowed [them] to calculate the fraction of the total
flux to CO2 that was channeled. [They] used an E. coli mutant that was engineered to take up 6-carbon sugar
phosphates constitutively." |
| Comments |
"The cells grew well on all carbon sources of interest to
[researchers], including the 6-carbon sugar phosphates, Glu6P,
Fru6P, and Fru1,6P2. The doubling times for Glu6P
and Fru6P were comparable with the doubling time
of the parental strain growing on glucose (Table 1).
Doubling times when grown on glycerol and Fru1,6P2
were considerably longer." |
| Entered by |
Uri M |
| ID |
110836 |