Monod substrate affinity constants (Ks) reported in the literature for different strains of E. coli growing on glucose as the only source of carbon and energy

Range 68µg/liter-99mg/liter Table - link
Organism Bacteria Escherichia coli
Reference Senn H, Lendenmann U, Snozzi M, Hamer G, Egli T. The growth of Escherichia coli in glucose-limited chemostat cultures: a re-examination of the kinetics. Biochim Biophys Acta. 1994 Dec 15 1201(3):424-36. P.425 left column top paragraph & P.433 table 4PubMed ID7803473
Primary Source [7] Shehata, T.E. and Marr, A.G. (1971) Effect of nutrient concentration on the growth of Escherichia coli. J. Bacteriol. 107, 210-216. [14] Schulze, K.L. and Lipe, R.S. (1964) Relationship between substrate concentration, growth rate, and respiration rate of E. coli in continuous culture. Arch. Mikrobiol. 48, 1-20. See pointers to refs on right of tablePubMed ID4935320, 14196722
Method For primary source [7] batch culture. For primary source [14] chemostat. For other studies see table 2nd column from left
Comments P.425 left column top paragraph: "For example, in the case of E. coli growing in glucose-limited culture, the Ks value in the Monod relationship is reported to vary by more than three orders of magnitude, i.e., from 68µg/liter [primary source 7] to 99 mg/liter [primary source 14] (see also Table 4)." P.433 left column top paragraph: "Two crucial problems can be identified when analysing the literature. Firstly, the scattering of the experimental data that one was able to obtain did not allow one to distinguish between competing models [refs 6,12,27,38]. Secondly, there is a considerable lack of consistency in the kinetic constants reported in the literature for a given microbe-substrate combination. This is exemplified in Table 4 for the case of the Monod saturation constant exhibited by E. coli for glucose which varies over more than three orders of magnitude."
Entered by Uri M
ID 111048