Monod substrate affinity constant (Ks) for strain ML30 of E. coli growing on glucose as the only source of carbon and energy

Value 53.4 µg/liter Range: Table - link µg/liter
Organism Bacteria Escherichia coli
Reference Senn H, Lendenmann U, Snozzi M, Hamer G, Egli T. The growth of Escherichia coli in glucose-limited chemostat cultures: a re-examination of the kinetics. Biochim Biophys Acta. 1994 Dec 15 1201(3):424-36. p.433 table 4PubMed ID7803473
Method Chemostat
Comments "Two crucial problems can be identified when analysing the literature. Firstly, the scattering of the experimental data that one was able to obtain did not allow one to distinguish between competing models [refs 6,12,27,38]. Secondly, there is a considerable lack of consistency in the kinetic constants reported in the literature for a given microbe-substrate combination. This is exemplified in Table 4 for the case of the Monod saturation constant exhibited by E. coli for glucose which varies over more than three orders of magnitude." Ks obtained from best fitting Monod model. Maximal growth rate [0.80hour^-1] obtained from curve fitting and not experimental values
Entered by Uri M
ID 111051