| Range |
Table - link nmole/μl cells
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, Rabinowitz JD. Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. Mol Syst Biol. 2013 Dec 3 9: 712. doi: 10.1038/msb.2013.65. Supplementary information p.8 table 2PubMed ID24301801
|
| Method |
"To quantify fluxes in central metabolism, [researchers] combined three
types of measurements (Figure 1A, see also Methods): (i)
uptake and excretion rates of major nutrients (glucose,
glutamine, and oxygen, including the fraction of oxygen consumed by oxidative phosphorylation as measured by
respiratory chain inhibition) and waste products (lactate,
glutamate, pyruvate, and alanine) (ii) cellular DNA, RNA,
protein, and fatty acid content (Supplementary Table 2)
together with cellular growth rate to determine the flux of
metabolic building blocks into biomass and (iii) steady-state
labeling of intracellular metabolites determined by LC-MS
when cells are fed media with [U-13C]-glucose or [U-13C]-
glutamine (Supplementary Figures 1–4)." |
| Comments |
iBMK=Immortalized baby mouse kidney epithelial cells |
| Entered by |
Uri M |
| ID |
110687 |