Diffusion coefficient of mRNAs within the nucleus measured by FRAP
| Value | 0.6 μm^2/s |
|---|---|
| Organism | Eukaryotes |
| Reference | Oeffinger M, Zenklusen D. To the pore and through the pore: a story of mRNA export kinetics. Biochim Biophys Acta. 2012 Jun1819(6):494-506. doi: 10.1016/j.bbagrm.2012.02.011. p.497 left column 2nd paragraphPubMed ID22387213 |
| Primary Source | (46) J.C.R. Politz, R.A. Tuft, K.V. Prasanth, N. Baudendistel, K.E. Fogarty, L.M. Lifshitz, et al., Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm, Mol. Biol. Cell 17 (2006) 1239–1249. (48) A. Calapez, H.M. Pereira, A. Calado, J. Braga, J. Rino, C. Carvalho, et al., The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive, J. Cell Biol. 159 (2002) 795–805. (52) R. Veith, T. Sorkalla, E. Baumgart, J. Anzt, H. Häberlein, S. Tyagi, et al., Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures, Biophys. J. 99 (2010) 2676–2685.PubMed ID16371503, 12473688, 20959109 |
| Method | Florescence recovery after photobleaching (FRAP) |
| Comments | "Measuring the behavior of the whole population of nuclear polyadenylated mRNA using a GFP-tagged nuclear poly(A)binding protein by florescence recovery after photobleaching (FRAP) suggested an mRNA diffusion coefficient of 0.6 µm^2/s, similar to measurements by florescence correlation spectroscopy approaches (FCS) that observed the diffusion of dT-labeled nuclear mRNA (1.0 µm^2/s) [primary sources]." |
| Entered by | Uri M |
| ID | 110665 |