Diffusion coefficient of dT-labeled nuclear mRNA measured by FCS

Value 1 μm^2/s
Organism Eukaryotes
Reference Oeffinger M, Zenklusen D. To the pore and through the pore: a story of mRNA export kinetics. Biochim Biophys Acta. 2012 Jun1819(6):494-506. doi: 10.1016/j.bbagrm.2012.02.011. p.497 left column 2nd paragraphPubMed ID22387213
Primary Source (46) J.C.R. Politz, R.A. Tuft, K.V. Prasanth, N. Baudendistel, K.E. Fogarty, L.M. Lifshitz, et al., Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm, Mol. Biol. Cell 17 (2006) 1239–1249. (48) A. Calapez, H.M. Pereira, A. Calado, J. Braga, J. Rino, C. Carvalho, et al., The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive, J. Cell Biol. 159 (2002) 795–805. (52) R. Veith, T. Sorkalla, E. Baumgart, J. Anzt, H. Häberlein, S. Tyagi, et al., Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures, Biophys. J. 99 (2010) 2676–2685.PubMed ID16371503, 12473688, 20959109
Method Florescence correlation spectroscopy approaches
Comments "Measuring the behavior of the whole population of nuclear polyadenylated mRNA using a GFP-tagged nuclear poly(A)binding protein by florescence recovery after photobleaching (FRAP) suggested an mRNA diffusion coefficient of 0.6 µm2/s, similar to measurements by florescence correlation spectroscopy approaches (FCS) that observed the diffusion of dT-labeled nuclear mRNA (1.0 µm^2/s) [primary sources]."
Entered by Uri M
ID 110666