Primary Source |
(1)D. Grünwald, R.H. Singer, In vivo imaging of labelled endogenous ß-actin mRNA during nucleocytoplasmic transport, Nature 467 (2010) 604–607 doi: 10.1038/nature09438. (50) D.Y. Vargas, A. Raj, S.A.E. Marras, F.R. Kramer, S. Tyagi, Mechanism of mRNA transport in the nucleus, Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 17008–17013. (51) Y. Shav-Tal, Dynamics of single mRNPs in nuclei of living cells, Science 304 (2004) 1797–1800. (52) R. Veith, T. Sorkalla, E. Baumgart, J. Anzt, H. Häberlein, S. Tyagi, et al., Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures, Biophys. J. 99 (2010) 2676–2685. (53) J.P. Siebrasse, R. Veith, A. Dobay, H. Leonhardt, B. Daneholt, U. Kubitscheck, Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin, Proc. Natl. Acad. Sci. 105 (2008) 20291–20296. (54) A. Mor, S. Suliman, S. Suliman, R. Ben-Yishay, R. Ben-Yishay, et al., Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells, Nat. Cell Biol. 12 (2010) 543–552.PubMed ID20844488, 16284251, 15205532, 20959109, 19074261, 20453848
|
Comments |
"So how long does it take an mRNA (or mRNP) to reach the nuclear
periphery? Considering that mRNAs move by Brownian motion, the
transit time of an mRNP across a typical mammalian cell nucleus
(7 µm in diameter) was calculated to be in the range of 2–6 min
[BNID 110668]. However, the recent observation that mRNPs can spend about
50% of their time ‘corralling’, might significantly increase their transit
time [primary sources]." For description of 'corral' see Görisch 2005 PMID 15830242, p.222 right column, 2nd paragraph:"In the MC [moving corral] model, the overall mobility of the particle
is described by a corral size of diameter rc in which the particle experiences confined diffusion with a diffusion coefficient Db." mRNP="Messenger RNP (messenger ribonucleoprotein)=mRNA with bound proteins. (wiki)" |