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Diffusion coefficient of mRNAs within the nucleus

Range	0.005 to >1 μm^2/s
Organism	Eukaryotes
Reference	Oeffinger M, Zenklusen D. To the pore and through the pore: a story of mRNA export kinetics. Biochim Biophys Acta. 2012 Jun1819(6):494-506. doi: 10.1016/j.bbagrm.2012.02.011. p.497 left column 2nd paragraphPubMed ID22387213
Primary Source	(1)D. Grünwald, R.H. Singer, In vivo imaging of labelled endogenous ß-actin mRNA during nucleocytoplasmic transport, Nature 467 (2010) 604–607 doi: 10.1038/nature09438. (45) J.C. Politz, R.A. Tuft, T. Pederson, R.H. Singer, Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells, Curr. Biol. 9 (1999) 285–291 (46) J.C.R. Politz, R.A. Tuft, K.V. Prasanth, N. Baudendistel, K.E. Fogarty, L.M. Lifshitz, et al., Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm, Mol. Biol. Cell 17 (2006) 1239–1249. (48) A. Calapez, H.M. Pereira, A. Calado, J. Braga, J. Rino, C. Carvalho, et al., The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive, J. Cell Biol. 159 (2002) 795–805. (50) D.Y. Vargas, A. Raj, S.A.E. Marras, F.R. Kramer, S. Tyagi, Mechanism of mRNA transport in the nucleus, Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 17008–17013. (51) Y. Shav-Tal, Dynamics of single mRNPs in nuclei of living cells, Science 304 (2004) 1797–1800. (52) R. Veith, T. Sorkalla, E. Baumgart, J. Anzt, H. Häberlein, S. Tyagi, et al., Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures, Biophys. J. 99 (2010) 2676–2685. (53) J.P. Siebrasse, R. Veith, A. Dobay, H. Leonhardt, B. Daneholt, U. Kubitscheck, Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin, Proc. Natl. Acad. Sci. 105 (2008) 20291–20296. (54) A. Mor, S. Suliman, S. Suliman, R. Ben-Yishay, R. Ben-Yishay, et al., Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells, Nat. Cell Biol. 12 (2010) 543–552.PubMed ID20844488, 10209094, 16371503, 12473688, 16284251, 15205532, 20959109, 19074261, 20453848
Comments	"The actual speed at which mRNAs diffuse within the nucleus is still up for discussion. A number of studies using a variety of imaging approaches resulted in different estimates that vary considerably from each other (from 0.005 µm^2/s up to more than 1 µm^2/s) [primary sources]. The main reason for these discrepancies is most likely technical. Interestingly, there is a large discrepancy in the values of the estimated diffusion coefficient between studies that used single molecule tracking (SMT) techniques to measure diffusion, but surprisingly little between studies that extracted their values measuring the behavior of total nuclear mRNAs by labeling all mRNAs simultaneously."
Entered by	Uri M
ID	110664