| Value |
26
steps/revolution
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Sowa Y, Rowe AD, Leake MC, Yakushi T, Homma M, Ishijima A, Berry RM. Direct observation of steps in rotation of the bacterial flagellar motor. Nature. 2005 Oct 6 437(7060):916-9. Abstract & p.917 right column top, 2nd and bottom paragraphs & p.918 left column top paragraphPubMed ID16208378
|
| Method |
"[Researchers] detected
rotation by back-focal-plane (BFP) interferometry of 500-nm diameter
polystyrene beads attached to spontaneously sticky flagellar
filaments of E. coli, as described previously [ref 16], or by high-speed
video recording of 200-nm fluorescent beads attached in the same
way (Fig. 1b, c, inset)." |
| Comments |
"[Researchers] observe 26 steps per revolution, which is consistent with
the periodicity of the ring of FliG protein, the proposed site of
torque generation on the rotor [refs 7,8]...The peak at 26 per revolution in the spectrum corresponds
to steps of 13.8° and indicates that successive revolutions
show the same stopping angles." |
| Entered by |
Uri M |
| ID |
110614 |