| Value |
190
molecules/cell
Range: ±80 molecules/cell
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Leake MC, Chandler JH, Wadhams GH, Bai F, Berry RM, Armitage JP. Stoichiometry and turnover in single, functioning membrane protein complexes. Nature. 2006 Sep 21 443(7109):355-8. p.357 left column top paragraphPubMed ID16971952
|
| Method |
"[Researchers] estimated the total number of GFP–MotB molecules per motor
by dividing I 0
m by IGFP for each trace (Supplementary Fig. 5c). The
reduced uncertainty of this estimate (22±6, ±s.d.) as compared
with the ratio of peaks in Supplementary Fig. 5a and b (19±8)
reflects covariation of Iom and IGFP, as expected for variations due
to TIRF [total
internal reflection fluorescence] intensity. Dividing the initial membrane component of background intensity by IGFP gave an average of 0.052±0.022
molecules per pixel. We estimated the cell-surface area as
3,700±500 pixels thus, the total number of non-motor GFP–MotB
molecules per cell is 190±80." |
| Entered by |
Uri M |
| ID |
109770 |