Number of GFP–MotB per motor

Value 22 molecules/motor Range: ±6 molecules/motor
Organism Bacteria Escherichia coli
Reference Leake MC, Chandler JH, Wadhams GH, Bai F, Berry RM, Armitage JP. Stoichiometry and turnover in single, functioning membrane protein complexes. Nature. 2006 Sep 21 443(7109):355-8. p.357 right column 2nd paragraphPubMed ID16971952
Method "[Researchers] measured the number and dynamics of MotB molecules labelled with green fluorescent protein (GFP-MotB) in the motor by total internal reflection fluorescence microscopy."
Comments "Fluorophore counting indicated that there are 22±6 GFP–MotB molecules per motor. [Researchers] confirmed the accuracy of the counting by testing it against simulated TIRF [total internal reflection fluorescence] photobleach traces generated by the extended diffusion model (Supplementary Fig. 11)."
Entered by Uri M
ID 109773