| Value |
22
molecules/motor
Range: ±6 molecules/motor
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Leake MC, Chandler JH, Wadhams GH, Bai F, Berry RM, Armitage JP. Stoichiometry and turnover in single, functioning membrane protein complexes. Nature. 2006 Sep 21 443(7109):355-8. p.357 right column 2nd paragraphPubMed ID16971952
|
| Method |
"[Researchers] measured the number and dynamics of MotB molecules labelled with green fluorescent protein (GFP-MotB) in the motor by total internal reflection fluorescence microscopy." |
| Comments |
"Fluorophore counting indicated that there are 22±6 GFP–MotB
molecules per motor. [Researchers] confirmed the accuracy of the counting by
testing it against simulated TIRF [total
internal reflection fluorescence] photobleach traces generated by the
extended diffusion model (Supplementary Fig. 11)." |
| Entered by |
Uri M |
| ID |
109773 |