Maximal Met production rate allowed by MetE (Vmax)

Value 34000 Met/sec
Organism Bacteria Escherichia coli
Reference Li GW, Burkhardt D, Gross C, Weissman JS. Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell. 2014 Apr 24 157(3):624-35. doi: 10.1016/j.cell.2014.02.033. p.631 right column bottom paragraphPubMed ID24766808
Method "[Researchers] ... compared the rate of Met consumption with the maximum velocity (Vmax) for its biosynthetic pathway. For each reaction in the pathway, [they] calculated Vmax by multiplying the enzyme abundance [they] determined by its published turnover number (kcat) (Schomburg et al., 2002)."
Comments "The Vmax varies by more than one order of magnitude among the reactions in Met biosynthesis, suggesting that most reactions do not operate at saturating substrate concentration. The last step that is catalyzed by MetE has among the smallest Vmax (Figure 7A), suggesting that it may be a bottleneck in this pathway. Remarkably, [researchers] found that the maximal Met production rate allowed by MetE (Vmax, 34,000/sec per cell) matches the Met consumption rate (31,000 Met/sec BNID 110446)." MetE="Methionine Synthase (MetE)"
Entered by Uri M
ID 110447