Maximal velocity (Vmax) of ATP synthase at 25°C

Value ~230 Revolutions/sec
Organism Bacteria Escherichia coli
Reference Ueno H, Suzuki T, Kinosita K Jr, Yoshida M. ATP-driven stepwise rotation of FoF1-ATP synthase. Proc Natl Acad Sci U S A. 2005 Feb 1 102(5):1333-8PubMed ID15668386
Method Rotation was probed with an 80-nm bead attached to the ring of c subunits in the immobilized FoF1 and recorded with a submillisecond fast camera.
Comments The rotation rates at various ATP concentrations obeyed the curve defined by a Km of approximately 30 microM and a Vmax of approximately 350 revolutions per second (at 37 degrees C). Researchers observed rotations at 25°C and 45°C and obtained the Vmax rotation rates of ˜230 and ˜650 rps, respectively. Therefore, extrapolated rotation rate at 60°C, an optimum growth temperature of Bacillus PS3, can reach ˜1,600 rps.
Entered by Uri M
ID 104894