Fraction of protein synthesis capacity devoted to MetE in media devoid of Met

Range ~8 %
Organism Bacteria Escherichia coli
Reference Li GW, Burkhardt D, Gross C, Weissman JS. Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell. 2014 Apr 24 157(3):624-35. doi: 10.1016/j.cell.2014.02.033. p.632 left column 2nd paragraphPubMed ID24766808
Method "In order to understand the amount of MetE expressed in the medium without Met, [researchers] constructed a quantitative model to predict the optimal level of MetE and growth rate. The model considers the cost and benefit of MetE synthesis on growth rate. The cost function is based on previous observations that synthesis of excess proteins competes with that of new ribosomal proteins, which in turn leads to slower growth rate (Scott et al., 2010)."
Comments "MetE is a large and slow enzyme, whose production consumes 8% of the total protein synthesis capacity in media devoid of Met." At 27min doubling time
Entered by Uri M
ID 110448