| Value |
31000
Met/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Li GW, Burkhardt D, Gross C, Weissman JS. Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell. 2014 Apr 24 157(3):624-35. doi: 10.1016/j.cell.2014.02.033. p.631 right column 2nd paragraphPubMed ID24766808
|
| Method |
"[Researchers] focused on the well-characterized L-Met biosynthetic
pathway for E. coli grown in media devoid of Met. [They] first calculated
the cellular demand for this pathway (31,000/sec Met per
cell), i.e., the rate of Met consumption by protein synthesis, by
summing up the absolute rates of protein synthesis [they] determined
for each protein multiplied by the number of Met residues
in that protein." |
| Comments |
"The other major pathway that consumes Met,
which is the synthesis of S-adenosyl-L-Met, was estimated
to contribute to a small fraction of the overall flux (Feist et al.,
2007) (see also Extended Experimental Procedures)." |
| Entered by |
Uri M |
| ID |
110446 |