Kcat (turnover rate) of activation of isoleucine by Leu tRNA Synthetase

Value 18 Sec^-1
Organism Bacteria Escherichia coli
Reference Chen JF, Guo NN, Li T, Wang ED, Wang YL. CP1 domain in Escherichia coli leucyl-tRNA synthetase is crucial for its editing function. Biochemistry. 2000 Jun 6 39(22):6726-31. Table - link PubMed ID10828991
Method Construction of a Plasmid Containing the Gene Encoding His6-Tagged LeuRS-B. Cloning and Expression of the Gene Encoding CP1Leu. Assay of Enzyme ActiVity. Circular Dichroism (CD) Spectroscopy.
Comments ATP-PPi exchange kinetics of enzymes were determined in the presence of 0.02-0.2 mM leucine and 5-50 mM methionine or isoleucine. All the data in this table were the average values with a variation of <10% from three independent determinations. The discrimination factors were calculated from the equation DMet or Ile ) [(kcat/Km)Leu]/[(kcat/Km)Met or Ile]. For value of 0.07 sec^-1 in human mitochondrial LeuRS see BNID 104979
Entered by Uri M
ID 104981