Intracellular Glutamate concentration in glucose-fed, exponentially growing E. coli

Value 96 mM Range: Table - link mM
Organism Bacteria Escherichia coli
Reference Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9 p.594 table 1PubMed ID19561621
Method Researchers quantified metabolites by Liquid chromatography-mass spectrometry (LC-MS/MS) using an isotope ratio-based approach (8). As isotope-labeled standards for many metabolites are not available, they used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (25). This enabled the use of commercially available unlabeled compounds as internal standards.
Comments The total observed intracellular metabolite pool was approximately 300 mM.A small number of metabolites dominate the metabolome on a molar basis, with glutamate being the most abundant.
Entered by Uri M
ID 104691