| Range |
Cell wet weight 1.7g/L: cell dry weight 0.39g/L
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Glazyrina J, Materne EM, Dreher T, Storm D, Junne S, Adams T, Greller G, Neubauer P. High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor. Microb Cell Fact. 2010 May 30 9: 42. doi: 10.1186/1475-2859-9-42. p.4 left column 5th paragraphPubMed ID20509968
|
| Method |
P.4 left column 5th paragraph: "Growth of the cultures was followed by
measuring the light absorbance at 600 nm (OD600), by measuring the cell wet weight, and by determination of
the cell dry weight. Therefore 1 ml cell suspension was centrifuged in pre-dried and pre-weighed 1 ml test tubes at 13000 × g for 5 min. After removal of the supernatant, the samples were measured for cell wet weight and then dried at 80°C for at least 24 h." |
| Comments |
P.4 left column 5th paragraph: "An OD600 of 1 resulted in a cell wet weight of 1.7 g/L and 0.39 g/L of dry weight." E.coli strain(s): E. coli BL21 and BL21(DE3) and recombinant strain E. coli RB791 pAdh. |
| Entered by |
Uri M |
| ID |
109836 |