Ratio cdw [g/L]/OD600

Value 0.33 g/liter
Organism Bacteria Escherichia coli
Reference Sauer U, Lasko DR, Fiaux J, Hochuli M, Glaser R, Szyperski T, Wüthrich K, Bailey JE. Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism. J Bacteriol. 1999 Nov181(21):6679-88. p.6680 right column 4th paragraphPubMed ID10542169
Method P.6680 right column 3rd paragraph: "For cellular dry weight (cdw) determination, a known volume of fermentation broth was centrifuged for 10 min in preweighed glass tubes at 4°C and 3,000 × g, washed once with water, and dried at 90°C for 24 h to a constant weight."
Comments P.6680 right column 4th paragraph: "A predetermined correlation factor (OD600, 0.33) was used to convert the OD600 values into cell concentrations for the calculation of specific conversion rates." E.coli strains: K12 MG1655 and knock out strains.
Entered by Uri M
ID 109838