Cell dry mass concentration for culture with OD600 of 1.0

Value 0.47 gDCW/L Range: ±0.03 gDCW/L
Organism Bacteria Escherichia coli
Reference Akhtar MK, Jones PR. Construction of a synthetic YdbK-dependent pyruvate:H2 pathway in Escherichia coli BL21(DE3). Metab Eng. 2009 May11(3):139-47 doi: 10.1016/j.ymben.2009.01.002. p.142 left column 2nd paragraphPubMed ID19558967
Method Abstract: "A synthetic pyruvate:H(2) pathway was constructed in Escherichia coli BL21(DE3) by co-expression of six proteins: E. coli YdbK, Clostridium pasteurianum [4Fe-4S]-ferredoxin, and Clostridium acetobutylicum HydF, HydE, HydG, and HydA. The effect of cofactor addition and host strain on H(2) yield and fermentation product accumulation was studied, together with in vitro reconstitution of the entire pathway."
Comments P.142 left column 2nd paragraph: "The relationship between OD600 and dry cell weight (DCW) for BL21 (DE3)grown aerobically in MOPS [3-(N-morpholino)propanesulfonic acid] minimal media (0.5% (w/v)glucose) was determined (an OD600 of 1.0 was equivalent to 0.47±0.03 g(dry cell weight)L^-1) as described by Sonderegger and Sauer (2003 [PMID 12676674])."
Entered by Uri M
ID 107924