Dry weight per OD460 unit of culture mass in different media

Range 172 glucose plus amino acids medium: 173 glycerol medium µg/OD460 unit/ml
Organism Bacteria Escherichia coli
Reference Brunschede H, Dove TL, Bremer H. Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein. J Bacteriol. 1977 Feb129(2):1020-33. p.1021 right column 4th paragraphPubMed ID320174
Method "The bacterial strain used was Escherichia Coli B/r (ATCC 12407). Cultures were grown at 37°C in minimal C medium (ref 15) supplemented with either 0.45% sodium succinate or 0.2% glucose or 0.2% glucose plus 20µg of each of the 20 standard L-amino acids per ml. Experimental cultures were inoculated by at least a 300-fold dilution of a fresh overnight culture containing the same nutritional supplements as the experimental culture. Growth was measured as the increase in the concentration of cell mass (absorbance at 460nm [A460] see below). The doubling times of the bacteria growing in the described media are listed in Table 1 (µ=Doublings per hour)."
Comments "The dry weight of (washed) bacteria was determined to be 172 and 173µg per A460 unit for bacteria grown in glucose plus amino acids or glycerol medium, respectively."
Entered by Uri M
ID 106437