Number of stators in bacterial flagellar motor
| Range | 8 to 16 stators/motor |
|---|---|
| Organism | Unspecified |
| Reference | Leake MC, Chandler JH, Wadhams GH, Bai F, Berry RM, Armitage JP. Stoichiometry and turnover in single, functioning membrane protein complexes. Nature. 2006 Sep 21 443(7109):355-8. p.355 left column bottom paragraphPubMed ID16971952 |
| Primary Source | [4] Block, S. M. & Berg, H. C. Successive incorporation of force-generating units in the bacterial rotary motor. Nature 309, 470–-472 (1984). [5] Blair, D. F. & Berg, H. C. Restoration of torque in defective flagellar motors. Science 242, 1678–-1681 (1988). [13] Berry, R. M., Turner, L. & Berg, H. C. Mechanical limits of bacterial flagellar motors probed by electrorotation. Biophys. J. 69, 280–-286 (1995). [14] Khan, S., Dapice, M. & Reese, T. S. Effects of mot gene expression on the structure of the flagellar motor. J. Mol. Biol. 202, 575–-584 (1988). [15] Reid, S. W. et al. The maximum number of torque-generating units in the flagellar motor of Escherichia coli is at least 11. Proc. Natl Acad. Sci. USA 103, 8066–-8071 (2006).PubMed ID6374467, 2849208, 7669906, 3050128, 16698936 |
| Method | "[Investigators] replaced genomic motB in E. coli with gfp–motB to express GFP–MotB in wild-type amounts (see Methods)." |
| Comments | "The bacterial flagellar motor is ideal for examining a single protein complex in vivo rotation of the whole cell when the filament is attached to a surface (Fig. 1) is an instantaneous indicator of motor function. Previous research indicates that a motor has 8–16 stators (primary sources), each containing two copies of MotB and four copies of MotA (16)." |
| Entered by | Uri M |
| ID | 109768 |