Dissociation rate of GFP–MotB between membrane pool and motor

Value 0.04 sec^-1 Range: ±0.02 sec^-1
Organism Bacteria Escherichia coli
Reference Leake MC, Chandler JH, Wadhams GH, Bai F, Berry RM, Armitage JP. Stoichiometry and turnover in single, functioning membrane protein complexes. Nature. 2006 Sep 21 443(7109):355-8. p.357 right column top paragraphPubMed ID16971952
Method Fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP)
Comments "Fitting all of the FRAP and FLIP data to an extended diffusion model, incorporating binding and unbinding at the motor and bleaching in the TIRF field (Supplementary Methods 7), gave a dissociation rate at the motor of 0.04±0.02sec^-1"
Entered by Uri M
ID 109772