Normalized extracellular aqueous volume (Vex) for E. coli BL21(DE3)
| Value | 3.6 μL/mg |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Wang, Lei, Zhou, Yongjin J., Ji, Debin, Zhao, Zongbao K., An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells, Journal of Microbiological Methods (2013), doi: 10.1016/j.mimet.2013.02.006 p.7 top paragraphPubMed ID23481146 |
| Method | Determining the Vex value by using NAD+ as the probe |
| Comments | "Vex values for E. coli BL21(DE3) and A. tumefaciens AGL1 were estimated to be 45.3 µL and 168.3 µL, respectively. Because DCW of E. coli BL21(DE3) and A. tumefaciens AGL1 were determined to be 12.7 mg and 45.3 mg, the normalized Vex values were 3.6 µL·mg–1 and 3.7 µL·mg–1, respectively." |
| Entered by | Uri M |
| ID | 108811 |