Intracellular aqueous volume (Vin)

Value 2.3 μL per mg of dry cell weight (DCW)
Organism Unspecified
Reference Wang, Lei, Zhou, Yongjin J., Ji, Debin, Zhao, Zongbao K., An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells, Journal of Microbiological Methods (2013), doi: 10.1016/j.mimet.2013.02.006 p.3 top paragraphPubMed ID23481146
Primary Source Bennett BD, Yuan J, Kimball EH, Rabinowitz JD. Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach. Nat Protoc. 2008 3(8):1299-311. doi: 10.1038/nprot.2008.107. AND Brown AF, Dunn GA. Microinterferometry of the movement of dry matter in fibroblasts. J Cell Sci. 1989 Mar92 ( Pt 3):379-89. AND Loferer-Krössbacher M, Klima J, Psenner R. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Appl Environ Microbiol. 1998 Feb64(2):688-94.PubMed ID18714298, 2592444, 9464409
Comments "the Vin value has been approximated as 2.3 µL per mg of dry cell weight (DCW), or more roughly, referred as the volume of the whole cell (primary sources)."
Entered by Uri M
ID 108810