Results of cellular volume determination by different methods

Range Table - link μL/mg
Organism prokaryote
Reference Wang, Lei, Zhou, Yongjin J., Ji, Debin, Zhao, Zongbao K., An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells, Journal of Microbiological Methods (2013), doi: 10.1016/j.mimet.2013.02.006 p.16 table 1PubMed ID23481146
Primary Source Bennett BD, Yuan J, Kimball EH, Rabinowitz JD. Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach. Nat Protoc. 2008 3(8):1299-311. doi: 10.1038/nprot.2008.107. AND Loferer-Krössbacher M, Klima J, Psenner R. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Appl Environ Microbiol. 1998 Feb64(2):688-94.PubMed ID18714298, 9464409
Method See methods on left of table
Comments "[researchers] estimated the cell volume according to the equation W = 435 × Vin^0.86 (Loferer–Krossbacher, et al., 1998), and obtained a Vin value of 3.6 µL·mg–1, which is 89% higher than the Vin value determined by [their] method (Table 1)."
Entered by Uri M
ID 108817