Normalized intracellular aqueous volume (Vin) for E. coli BL21(DE3)

Value 6.7E-10 μL/cell
Organism Bacteria Escherichia coli
Reference Wang, Lei, Zhou, Yongjin J., Ji, Debin, Zhao, Zongbao K., An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells, Journal of Microbiological Methods (2013), doi: 10.1016/j.mimet.2013.02.006 p.8 top paragraphPubMed ID23481146
Method To determine the Vin value, standard NAD+ solutions were prepared in 0.2 M HCl. This solution was used to rupture cells and extract the intracellular NAD+
Comments "...the Vin values of E. coli BL21(DE3) and A. tumefaciens AGL1 samples were determined to be 24.2 µL and 80.2 µL, and the normalized Vin values were 1.9 µL·mg–1 and 1.8 µL·mg–1, respectively. As 1 OD600 equals to 7.8 ×10^8 cell·mL–1 for E. coli culture (Volkmer and Heinemann, 2011), the Vin value could also be normalized as 6.7×10^–10 µL/cell."
Entered by Uri M
ID 108815