Transcription elongation rate with chloramphenicol

Value 27 nts/sec Range: Table - link nts/sec
Organism Bacteria Escherichia coli
Reference Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.505 left column 2nd paragraph & table 1PubMed ID20413502
Method P.505 left column 2nd paragraph: "To calculate the elongation rate, the Escherichia coli culture was induced with IPTG, and the time that elapsed between the appearance of a specific hybridization signal from probes complementary to the 5' and 3' segments of the lacZ transcript was determined by dotblot hybridization (Fig. 1 and fig. S1) (refs 5, 6)."
Comments P.505 left column 2nd paragraph: "In the absence of Cm (chloramphenicol), the transcription elongation rate was determined to be 42 nucleotides (nt) per second under specified growth conditions, matching a translation elongation rate of 14 amino acids per second (Table 1 and fig. S2). However, Cm reduced the transcription elongation rate to 27 nt/s (Fig. 1B), which matched a reduced translation rate of nine amino acids per s (Table 1 and fig. S2). This result suggests that the ribosome controls the rate of RNAP propagation."
Entered by Uri M
ID 108489