Transcription and translation rates of individual genes

Range Table - link
Organism Bacteria Escherichia coli
Reference Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.506 table 2PubMed ID20413502
Primary Source 29) Varenne S, Buc J, Lloubes R, Lazdunski C. Translation is a non-uniform process. Effect of tRNA availability on the rate of elongation of nascent polypeptide chains. J Mol Biol. 1984 Dec 15 180(3):549-76. AND (6) Vogel U, Jensen KF. The RNA chain elongation rate in Escherichia coli depends on the growth rate. J Bacteriol. 1994 May176(10):2807-13.PubMed ID6084718, 7514589
Method "To calculate the elongation rate, the Escherichia coli culture was induced with IPTG, and the time that elapsed between the appearance of a specific hybridization signal from probes complementary to the 5' and 3' segments of the lacZ transcript was determined by dotblot hybridization (Fig. 1 and fig. S1) (refs 5, 6)."
Comments "Remarkably, transcription of rplB-tufA matched its fast translation rate and was more than 1.5 times as fast as that of srb4 (Fig. 1D and Table 2). Moreover, genes carrying an intermediate number of rare codons (lacZ and infB) predictably displayed intermediate rates of transcription (Table 2). Thus, codon usage is a key determinant of transcription rate."
Entered by Uri M
ID 108490