Transcription elongation rate in CH184 strain with streptomycin (Sm)

Value 30 nts/sec Range: Table - link nts/sec
Organism Bacteria Escherichia coli
Reference Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.505 table 1 and middle column top paragraphPubMed ID20413502
Method "To calculate the elongation rate, the Escherichia coli culture was induced with IPTG, and the time that elapsed between the appearance of a specific hybridization signal from probes complementary to the 5' and 3' segments of the lacZ transcript was determined by dotblot hybridization (Fig. 1 and fig. S1) (5, 6)."
Comments "The speed of transcription elongation in CH184 was remarkably slow (only 19 nt/s), but accelerated to 30 nt/s when Sm was added (Fig. 1C), which perfectly matched the translation elongation rates with and without Sm (Table 1)."
Entered by Uri M
ID 108492