Estimations of periplasmic volume from distributions of different solutes in suspensions of Salmonella typhimurium LT2

Range Table - link
Organism Bacteria Salmonella typhimurium
Reference Stock JB, Rauch B, Roseman S. Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem. 1977 Nov 10 252(21):7850-61 p.7855 table IIIPubMed ID334768
Comments P.7853 right column 2nd paragraph: "Results such as those presented in Fig. 3 indicated that, after correcting for material contained in the cytoplasm, the intracellular level of any low molecular weight neutral solute could be used to determine periplasmic volume. This conclusion was substantiated by measuring the distributions of several small solutes in addition to TMG [methyl β-D-thiogalactopyranoside] and sucrose. As shown in Table III, the same periplasmic volumes were obtained with mannitol, N-acetylglucosamine, sucrose, and TMG. In this experiment, a strain of S. typhimurium defective in its ability to transport sugars was used so as to minimize cytoplasmic uptake." See note above table
Entered by Uri M
ID 108473